Comparison of content of 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside and anthraquinon in different ploidy Radix Polygoni Multiflori

Objective: To investigate the content of 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside and anthraquinon in diploid and triploid Radix Polygoni Multiflori.

Methods: 4 batches of Radix Polygoni Multiflori were collected from different districts. The content of 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside and anthraquinon in these samples were determined at 320 nm and 254nm wave length by HPLC with Inertsil ODS-3 C18 pillar and acetomitrile: aqua (25:75), methanol: 0.1% phosphoric (85:15) respectively as the mobile phase.

Results: The maximum content of 2,3,5,4'-tetrahydroxystilbene-2-0-beta-D-glucoside was diploid Radix Polygoni Multiflori from Deqing Guangdong. The maximum ratio of total anthraquinon was triploid Radix Polygoni Multiflori from Jinxi Guangxi reached 85%.

Conclusions: The content of anthraquinon varies greatly in the samples from the different producing areas.