Pathway of dephosphorylation of myo-inositol hexakisphosphate by a novel phytase from Candida krusei WZ-001.

myo-Inositol hexakisphosphate (InsP6) was hydrolyzed by a novel phytase purified from Candida krusei. The chemical structures of the product (InsP(1-5)) were identified by an NMR technique. The data indicate that the products from the enzyme catalytic reaction in sequence are D/L-Ins(1,2,4,5,6)P5, D/L-Ins(1,2,3,5,6)P5, D/L-Ins(1,2,5,6)P4, D/L-Ins(1,2,5)P3, D/L-Ins(1,2)P2 and D/L-Ins(2)P1.