Clinical significance of lymph vessel density marked by D2-40 in laryngeal squamous cell carcinomas

Objective: To study the relationship between lymphatic vessel density and clinicopathological features of laryngeal squamous cell carcinoma.

Methods: The lymph vessels, 40 specimens of LSCC and normal mucosa, were quantitated by SABC immunohistochemistry staining with lymphatic endothelial marker oncofetal antigen M2A Monoclonal antibody (D2-40).

Results: The density of peritumoral D2-40(+) vessels in the LSCC group was higher than that of normal mucosa one (P<0.01). The density of peritumoral D2-40(+) vessels in T3/4 group was higher than that of T1/2 group (P<0.05). The density of peritumoral D2-40(+) vessels in the neck lymph nodal metastasis group was higher than that without neck lymph nodal metastasis group (P<0.05). The density of peritumoral D2-40(+) vessels in the supraglottic group was higher than that of glottic group (P<0.01). The density of peritumoral D2-40(+) vessels among high, middle, poor differentiation group were no significant difference (P>0.05).

Conclusions: Tumor lymph vessel mainly means peritumoral lymph vessel. The density of peritumoral lymph vessel marked with D2-40 in laryngeal carcinoma tissues was significantly correlated with progression and invasion of tumor.