Bladder smooth muscle cells interaction and proliferation on PCL/PLLA electrospun nanofibrous scaffold.

Objective: Numerous synthetic materials have been used for the bladder reconstruction; of which, nano-structured scaffolds are used as relevant implant to the bladder tissue-engineering. The aim of this study was to investigate the capacity of Poly ε-caprolactone/poly-L-lactide acid (PCL/PLLA) nanofibrous scaffold, in supporting the maintenance and attachment of the human bladder smooth muscle cells (BdSMCs).

Methods: In this study, BdSMCs were isolated by enzymatic digestion method. Then, cells were seeded on PCL/PLLA nanofibrous scaffolds. Thereafter, cell attachment and expansion were analyzed by Hematoxylin and Eosin staining (H&E), immunohistochemistry, and scanning electron microscopy (SEM). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay confirmed that the nano-structured scaffold supported and maintained normal cell viability without inducing cytotoxic events.

Results: H&E staining, immunohistochemistry, and SEM showed that BdSMCs were attached and expanded on PCL/PLLA nanofibrous scaffolds after 14 days. Cell viability of BdSMCs on PCL/PLLA nanofibrous scaffolds increased during 14 days.

Conclusions: Our results showed that the novel porous nanofibrous electrospun scaffold is a biocompatible structure for attachment and adhesion of BdSMCs. However, there is not enough information on the stimulating effect of this nanofiber on the cells. Therefore, further in-vivo studies seem required to confirm such a nanofiber to be used in the bladder tissue-engineering.