NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes.
Journal: Nature Methods
Published:
Abstract
NKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-5 locus of human embryonic stem cells (hESCs); NKX2-5(eGFP/w) hESCs facilitate quantification of cardiac differentiation, purification of hESC-derived committed cardiac progenitor cells (hESC-CPCs) and cardiomyocytes (hESC-CMs) and the standardization of differentiation protocols. We used NKX2-5 eGFP(+) cells to identify VCAM1 and SIRPA as cell-surface markers expressed in cardiac lineages.
Authors
David Elliott, Stefan Braam, Katerina Koutsis, Elizabeth Ng, Robert Jenny, Ebba Lagerqvist, Christine Biben, Tanya Hatzistavrou, Claire Hirst, Qing Yu, Rhys J Skelton, Dorien Ward Van Oostwaard, Sue Lim, Ouda Khammy, Xueling Li, Susan Hawes, Richard Davis, Adam Goulburn, Robert Passier, Owen W Prall, John Haynes, Colin Pouton, David Kaye, Christine Mummery, Andrew Elefanty, Edouard Stanley
Relevant Conditions