Association between adiponectin rs2241766, rs1501299 polymorphisms and the risk of colorectal cancer

Objective: To explore the association between adiponectin rs2241766, rs1501299 polymorphisms and gene-environment interaction and the risk of colorectal cancer(CRC).

Methods: Four hundred CRC patients confirmed by histopathology and 400 healthy controls were recruited in this study. Cases and controls were matched on age and gender. A well-designed questionnaire was used to collect the information of demography, lifestyle and dietary habit on the 400 case-control pairs. Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)was applied to detect the adiponectin rs2241766 and rs1501299 genotypes.

Results: Data from conditional logistic regression analysis showed that those carrying TG+GG genotype on rs2241766 having an increased risk compared to those that carrying TT genotype(OR = 1.354, 95%CI:1.004-1.827), and those that carrying GT+TT genotype on rs1501299 having an decreased risk when compared to those that carrying GG genotype(OR = 0.680, 95% CI:0.501-0.923), after adjusted by factors as CRC family history,BMI, sedentary time, red meat consumption, and tea-drinking habit. Data from generalized multifactor dimensionality reduction showed that the gene-environment interaction among rs2241766, rs1501299 and red meat consumption on the risk of CRC might be significant(P = 0.001). A significant dosage effect with an increasing number of risk genotypes was observed as the risk of CRC increased (χ(2) = 8.458, P = 0.004).

Conclusions: Both adiponectin rs2241766 and rs1501299 were associated with CRC risk and the two SNPs might have worked together with red meat consumption in affecting the CRC risk.