Electrospray ionization-collision-induced dissociation-tandem mass spectrometry study of lead complexes with deprotonated nucleobases.

The complexes between the lead cation and deprotonated nucleobases (and deprotonated nucleosides) are studied by using electrospray ionization-collision-induced dissociation-tandem mass spectrometry. It has been found that the deprotonated N9 atom is not the site of lead cation attachment. In ions [A - H + Pb]+ and [C - H + Pb]+, the lead cation is coordinated by the adenine N1 atom and cytosine  N3 atom and interaction between the lead cation and deprotonated amino groups seems very likely. Deprotonated thymine shows a higher affinity toward lead cation than deprotonated uracil. In the lead-nucleoside complexes lead cation interacts with a sugar moiety.