Ultra-Specific Isolation of Circulating Tumor Cells Enables Rare-Cell RNA Profiling.
Journal: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
Published:
Abstract
The clinical potential of circulating tumor cells (CTCs) in managing cancer metastasis is significant. However, low CTC isolation purities from patient blood have hindered sensitive molecular assays of these rare cells. Described herein is the ultra-pure isolation of CTCs from patient blood samples and how this platform has enabled highly specific molecular (mRNA and miRNA) profiling of patient CTCs.
Authors
Rhonda Jack, Meggie M Grafton, Danika Rodrigues, Maria Giraldez, Catherine Griffith, Robert Cieslak, Mina Zeinali, Chandan Kumar Sinha, Ebrahim Azizi, Max Wicha, Muneesh Tewari, Diane Simeone, Sunitha Nagrath