Multi-components determination by single reference standard and HPLC fingerprint analysis for Lamiophlomis rotata Pill.

A validated HPLC method was developed to evaluate the quality of Lamiophlomis rotata Pill combining the multi-components analysis by single reference standard with HPLC fingerprint analysis. Five bioactive components (shanzhiside methyl ester, loganin, 8-O-acetylshanzhiside methyl ester, forsythoside B and luteolin-7-O-β-D-glucopyranoside) were selected as markers to control the quality of L. rotata Pill. The results revealed that the chromatographic fingerprint method coupled with multi-components analysis provides an effective and feasible way to determine the components in L. rotata Pill.