Reprogram Murine Epiblast Stem Cells by Epigenetic Inhibitors.
Journal: Bio-Protocol
Published:
Abstract
Pluripotent stem cells in the naïve state are highly useful in regenerative medicine and tissue engineering. A robust reprogramming of the primed murine Epiblast Stem Cells (EpiSCs) to naïve pluripotency is feasible via chemical-only approach. This protocol described a method to reprogram murine EpiSCs by MM-401 treatment, which blocks histone H3K4 methylation by MLL1/KMT2A.
Authors
Hui Zhang, Yali Dou