Untargeted metabolomic analysis in non-fasted diabetic dogs by UHPLC-HRMS.

Introduction: We recently identified variances in serum metabolomic profiles between fasted diabetic and healthy dogs, some having similarities to those identified in human type 1 diabetes.

Objectives: Compare untargeted metabolomic profiles in the non-fasted state.

Methods: Serum from non-fasted diabetic (n = 6) and healthy control (n = 6) dogs were analyzed by liquid chromatography-high resolution mass spectrometry.

Results: Clear clustering of metabolites between groups were observed, with multiple perturbations identified that were similar to those previously observed in fasted diabetic dogs.

Conclusion: These findings further support the development of targeted assays capable of detecting metabolites that may be useful as biomarkers of canine diabetes.