Generation of a set of isogenic iPSC lines carrying all APOE genetic variants (Ɛ2/Ɛ3/Ɛ4) and knock-out for the study of APOE biology in health and disease.
Journal: Stem Cell Research
Published:
Abstract
APOE genotype is the strongest genetic risk factor for Alzheimer's Disease (AD). The low degree of homology between mouse and human APOE is a concerning issue in preclinical models currently used to study the role of this gene in AD pathophysiology. A key objective of ADAPTED (Alzheimer's Disease Apolipoprotein Pathology for Treatment Elucidation and Development) project was to generate in vitro models that better recapitulate human APOE biology. We describe a new set of induced pluripotent stem cells (iPSC) lines carrying common APOE variants (Ɛ2, Ɛ3, and Ɛ3/Ɛ4) and a knock-out isogenic to the parental APOE Ɛ4/Ɛ4 line (UKBi011-A).
Authors
Benjamin Schmid, Bjørn Holst, Christian Clausen, Lamiaa Bahnassawy, Peter Reinhardt, Margot H Bakker, Eva Díaz Guerra, Carlos Vicario, Pamela Martino Adami, Michaela Thoenes, Alfredo Ramirez, Klaus Fliessbach, Clara Grezella, Oliver Brüstle, Michael Peitz, Andreas Ebneth, Alfredo Cabrera Socorro
Relevant Conditions