The standardization of the in-vitro tetanus toxin neutralization test on Chinese hamster ovary cells

A test for the titration of B. pertussis toxin with antisera on Chinese hamster ovary (CHO) cells has been worked out. B. pertussis protective antigenic cell-free complex containing 48-54% of B. pertussis toxin has been used as antigen. The specificity of the effect of this complex on CHO cells has been confirmed in the toxicity neutralization test with antisera. CHO cells have been adapted to reagents and culture media made in the USSR. The titration of B. pertussis toxin and antisera on CHO cells did not require the use of highly purified antigen.