Limiting Dilution Tumor Initiation Assay: An In Vivo Approach for the Study of Cancer Stem Cells.
Journal: Methods In Molecular Biology (Clifton, N.J.)
Published:
Abstract
Cancer stem cells (CSCs) are a small subpopulation of self-renewing cancer cells that are present within tumors. Calculating the frequency of tumor-initiating cells is important in the assessment of the number of CSCs present in a cell population. In this chapter, we present a protocol developed for quantification of CSCs from breast cancer tumors that can be adapted to CSCs from other types of tumors.
Authors
Petra Den Hollander, Robiya Joseph, Suhas Vasaikar, Nick Kuburich, Abhijeet Deshmukh, Sendurai Mani
Relevant Conditions