The effect of 400 nm femtosecond laser and Zn(II)chlorin e6 methyl ester (Zn(II)e6Me) photosensitizer on disinfection of radicular dentin bonded to glass fiber post.

Objective: To assess and compare push-out bond strength (PBS) when canal disinfected with 5.25% NaOCl, 400 nm femtosecond laser, and natural PS activated by PDT (CP and Zn (II) Ce6 methyl ester) on bond efficacy of radicular dentin bonded to glass fiber post.

Methods: A sum of 40 non-traumatic, cautiously extracted with closed apex human mandibular premolars were collected and decoronated up to the cement-o-enamel junction. Canals of all specimens were cleaned and shaped using a Protaper universal NiTi system via a crown-down approach, then finally dried out and obturated with gutta-percha. Post alignment space was formed with peso reamers and sanitized. Then samples were randomly allocated into four groups as per the radicular dentin designated disinfection protocols. Group 1 disinfected with curcumin photosensitizer + EDTA, group 2: 5.25% NaOCl and EDTA, group 3: Zn (II) Ce6 methyl ester and EDTA and group 4 sanitized with 400 nm fs-laser + EDTA. GFP was cemented into the radicular canal space and specimens were placed in a thermocycler. Segmentation of the root was done at three levels (coronal, middle, and apical) and positioned in a universal testing machine for PBS analysis. Failure mode analysis was performed using a stereomicroscope and statistical analysis was executed by one-way ANOVA and Tukey multiple comparison tests at a statistically significant level of p < 0.05.

Results: The highest PBS was revealed by group 2 canal disinfected with 5.25% NaOCl +17% EDTA (control) at coronal (9.49 ± 1.42 MPa) and middle (7.545 ± 0.15 MPa) root levels. Whereas, the lowest PBS was displayed by group 3: Zn (II) Ce6 methyl ester +17% EDTA at coronal (6.31 ± 0.54 MPa) middle (4.85 ± 0.11 MPa) root levels respectively. Intergroup comparison presented that radicular dentin disinfected with 400 nm femtosecond laser + 17% EDTA substantiated comparable PBS result to group 2 (control) at all three root levels (p > 0.05). Likewise, root surface disinfection with Curcumin activated by PDT+17% EDTA unveiled no statistically significant result for specimens disinfected with Zn(II) Ce6 methyl ester +17% EDTA(p > 0.05). Furthermore, apical root segments in all groups showed a substantial decrease in PBS value and were comparable (p > 0.05).

Conclusions: The bond efficacy of glass fiber post to radicular dentin disinfected with 400 nm femtosecond laser has the potential to be used as an alternative to conventional sodium hypochlorite.