Comparative study of various antigens of D. filaria by disc electrophoresis

Somatic and functional antigens of Dictyocaulus filaria were comparatively studied by means of disk electrophoresis. Established were the protein fractions of the different antigens. The preliminary fractionation of the antigens on Sephadex G-200 made it possible (at a second concentration and separation through disk electrophoresis) to obtain fewer, but richer and better distinguishable fractions each one of which could be used alone as a specific antigen. The complement-fixation test, the indirect hemagglutination, and the latex agglutination reaction revealed that most specific and sensitive proved the antigen obtained after Stewart (II fraction), this method being more sensitive and specific, as compared with others. The c. f. test performed with this antigen gave consistently better results showing higher titers as against other antigens.