Molecular Detection, Seroprevalence and Biochemical Analysis of Lumpy Skin Disease Virus.

Lumpy skin disease (LSD) is a transboundary viral disease caused by lumpy skin disease virus (LSDV), belonging to the Capripoxvirus genus and Poxviridae family. This study reports on the molecular detection, seroprevalence and biochemical analysis of samples from cattle infected with LSDV in Madhya Pradesh (MP) and Telangana. A total of 189 samples (116 blood, 26 tissue, 47 nasal swabs) were collected from MP during 2022-2023. Molecular detection was performed using conventional PCR targeting the P32 and fusion genes, while seroprevalence was assessed using an indirect ELISA kit on 184 serum samples collected from MP and Telangana between 2022 and 2024. Tissue samples showed a higher positivity rate (69.23%) for the P32 gene, while nasal swabs had a 6.38% positivity rate. The fusion gene was detected in 77.77% of tissue and 66.66% of nasal swab samples. The seroprevalence study revealed that 19.56% of serum samples were positive, with a higher prevalence of 86.11% in MP. Biochemical analysis indicated elevated levels of SGPT, SGOT, BUN, creatinine, albumin, globulin and the A/G ratio in LSDV-infected cattle, though these differences were not statistically significant. The study emphasizes that blood samples are not ideal for LSDV detection and the timing of serum sample collection plays a critical role in seroprevalence studies.