Borrelia miyamotoi DNA in a patient suspected of Lyme borreliosis.

Introduction and Objective. Manifestations of infection caused by Borrelia miyamotoi can mimic highly variable symptoms of Lyme borreliosis. The aim of the study was to detect DNA from B. miyamotoi samples from patients with suspected neuroborreliosis. Materials and Method. Samples of blood serum and cerebrospinal fluid (CSF) were collected from 133 patients. Diagnosis was established by the detection of specific antibodies to Borrelia burgdorferi sensu lato (s.l.) with ELISA and immunoblot. All Borrelia-positive samples were tested by nested PCR for the B. miyamotoi and B. burgdorferi s.l. DNA. Results. B. miyamotoi DNA was detected in the CSF of one (0.8%) patient. DNA of B. burgdorferi s.l. was not found in any samples. Conclusions. Detection of the B. miyamotoi in patients with central nervous system infections expand the development of knowledge on infections caused by Borrelia spirochetes.