Quick separation and enrichment of chlorogenic acid and its analogues by a high-efficient molecularly imprinted nanoparticles and evaluation of antioxidant and hypoglycemic activities.

Multiple interaction strategy to target was tried to use in the design of surface imprinting polymer. To validate this, active chlorogenic acid, a representative phenolic acid compound existing in many natural products, was selected as the template molecule and a magnetic molecularly imprinted nanoparticles (CGA-MMIPs) was synthesized. The characterizations indicated CGA-MMIPs was 20-50 nm, stable below 229.56 °C and had a saturation magnetic intensity of 17.90 emu/g. The prepared CGA-MMIPs exhibited high adsorption capacity (441.81 mg/g) and fast adsorption equilibrium for chlorogenic acid. It also was easy separation, high selectivity and good reusability, which was successfully used in quick separation of chlorogenic acid from Orthosiphon aristatus and Taraxacum mongolicum and Salvia miltiorrhiza. Moreover, the isolated substances possessed great antioxidant and hypoglycemic activities. These verified the strategy was useful and had huge prospects in the quick separation of chlorogenic acid or other phenolic acid compounds from natural products.