Colorimetric determination of Krebs Cycle dehydrogenases in mycelium of Actinomycetes (Streptomyces levoris)

A new procedure for colorimetric determination of the dehydrogenase activity in the mycelium of Streptomyces levoris was developed. The procedure is more precise, universal and adaptive. The novelty of the procedure is in the switching of the respiration chain to ascorbic acid that excludes the use of the respiration chain inhibitors. The adaptivity refers to the adjusting of the reaction to a particular strain by the reaction time and mycelium concentration in the reaction mixture. The procedure is technologically advantageous and handy. It provides simultaneous assay of a large number of samples of different type dehydrogenases.