Polarized populations of T helper cells in patients with chronic hepatitis B virus infection

Objective: To investigate the composition of Th1/Th2/Th3 cells in chronic HBV-infected individuals by determining the expression of IL-4/IFN-gamma/TGF-beta in single CD4+ T cell isolated from the peripheral blood mononuclear cells (PBMCs), and analyze the role of Th cells polarized population in chronic HBV-infection.

Methods: PBMCs from chronic HBV infected individuals were separated routinely, stimulated by PMA/Ionomycin/Monensin, and the production of IL-4/IFN-gamma/TGF-beta by CD4+ T cells in PBMCs was determined by FACS analysis.

Results: The percentage of IFN-gamma-producing T cells, IL-4-producing T cells and TGF-beta producing T cells ranged from 2.3%-18.6%, 1.1%-8.7% and 0.7%-7.1%, respectively, in CD4+ cells from non-infected individuals. The majority of CD4+ T cells from PBMCs in chronic HBV-infected individuals were Th0 cells. The proportion of Th1 cells increased significantly with the hepatic inflammation activity, and the proportion in active period of chronic hepatitis B was higher than that in nonactive period (P < 0.05). The percentage of Th2 cells in CD4+ T cells from HBV-infected individuals did not differ significantly (P > 0.05), but it was higher than controls (P < 0.05). The percentage of Th3 cells in CD4+ T cells from AsC group was higher than that in CHB group and control (P < 0.05).

Conclusions: Th1 phenotype cytokine are correlated with hepatic inflammation activity of chronic hepatitis B. Th2 cells may be associated with the persistence of HBV infection. Th3 cells cooperating with Th2 cells exert negative immunoregulating action and may be associated with the immune tolerance state of chronic HBV infection.