Expression of leucocyte cell-surface antigens on colon cancer cell line HR8348
Journal: Zhejiang Da Xue Xue Bao. Yi Xue Ban = Journal Of Zhejiang University. Medical Sciences
Published:
Abstract
Objective: To define the immune phenotype of colon cancer cells.
Methods: Using a panel of 40 anti-human monoclonal antibodies (MoAbs), the cells of colon cancer HR8348 were analyzed with three-color flow cytometry after direct immunofluorescent staining.
Results: HR8348 cell line did not express CD2, CD3, CD4, CD5, CD7, CD8, TCR, CD10, CD11b, CD14, CD16, CD19, CD22, CD25, CD28, SmIg, CD33, CD35, CD36, CD41a, CD45, CD45RA, CD45RO, CD56, CD61, CD64, CD66b, CD69, CD71, CD117, CD122 and P-glycoprotein but expressed CD13, CD15, CD20, CD38, CD95 and HLA-DR.
Conclusion: The results demonstrate that colon cancer cell line HR8348 shares some antigenic determinants with leucocyte lineage.
Authors
Shi-long Yang, Yon-min Tang, Hong-qiang Shen, Bo-qin Qian, Hua Song, Shu-wen Shi, Bo-tao Ning, Ying-hu Chen, Wei-qun Xu
Relevant Conditions