We compared the ability to discriminate between healthy and glaucomatous eyes of three optical imaging devices in 140 eyes from 140 subjects. No statistically significant differences were found among the AUCs of these parameters. However, AUCs were significantly higher in OCT and HRT parameters than most of GDx VCC ones. Thus, structural parameters assessed by the optical imaging devices are useful to discriminate glaucomatous damage, but showed no significant difference among the best parameters from HRT, OCT or GDx VCC.