A case of porcine malignant hyperthermia (MH) is reported, which occurred during a cardiological animal experiment under general anaesthesia using isoflurane as inhalational anaesthetic. Initially, tachycardia (238 bpm) and a sudden increase of end-exspiratory pCO2 were noticed. Discontinuation of isoflurane followed by administration of dantrolene (7 mg/kg body weight) led to a rapid recovery. Two days later, no difference in clinical state was apparent in comparison with the remaining swine. After regular euthanasia, acute muscle necrosis was seen macroscopically in back and glutaeal muscles, which was confirmed by histological examination. The rise of end-tidal pCO2 was the striking symptom thus allowing the detection of the incipient malignant hyperthermia. Early onset of dantrolene therapy is shown to be sufficient in termination of this hyperacute complication, and may prevent its deleterious outcome. Therefore, the administration of dantrolene should be taken into account in case of porcine MH, especially in time-consuming and costly series of animal experiments.