An adolescent female with Reiter's disease and positive for HLA-B27 is presented. Chlamydia trachomatis was cultured from the eye and Ureaplasma urealyticum and Mycoplasma were isolated from the urogenital tract. Joint and serologic evidence for Chlamydia and Ureaplasma were present. A brief review of Reiter's disease in children and adolescents is discussed.