Metastatic involvement of the spleen is uncommon. Lymphoma is the most common cause of primary and secondary malignant lesions of the spleen, whereas splenic metastases from carcinoma are rare and usually occur in a setting of concomitant carcinomatosis with melanoma, breast, ovary and lung accounting for the principal primary tumors. We report a case of an isolated, focal splenic defect demonstrated by Tc-99m-sulfur colloid scintigraphy which was the only visual evidence of metastatic adenocarcinoma of the colon. To our knowledge this unusual cause of a focal splenic defect on liver-spleen scintigraphy has not been previously described.