Establishment and biological characteristics of vincristine: resistant cell line from human squamous cell carcinoma

Objective: To establish a VCR-resistant cell line from human squamous cell carcinoma.

Methods: The cell line Tca8113 was utilized. The VCR concentration was increased gradually in consecutively passage of cultures.

Results: A VCR-resistant cell line Tca8113/V100 from Tca8113 was established, the cells grew steadily in 100 mumol/L medium and with 19.2 times of resistance to VCR. The cell line was also resistant to ADM, PYM, CDDP, MTX, 5-Fu at different degrees. The doubling time of the cells was 37.2 hours and showed microscopically and ultrastructurally typical squamous cell carcinoma when inoculated in nude mice. Blot hybridization showed the cells had increased espression of MDR-1mRNA.

Conclusions: These results indicate that the cell line has multidrug resistant ability, and the reason for this may be over expression of MDR-1 gene. This cell line provides an important model for the study on drug-resistant cell both in cellular and molecular level, and on the mechanism of drug resistance.