Establishment and drug-resistance gene expression of cisplatin-resistant cell line Tca8113/CDDP

Objective: To establish a CDDP-resistant cell line from human tongue squamous cell carcinoma cell line Tca8113 and to compare the drug-resistance gene expression between them.

Methods: The concentration of CDDP added to Tca8113 cells was increased stepwise to induce the CDDP-resistance. The index of drug-resistance was estimated by MTT assay. The expression of MDR-1, MRP, GST-pi and Bcl-2 was measured by RT-PCR optical density OD.

Results: The CDDP-resistant cell line, Tca8113/CDDP was established, whose drug-resistance index was 9.2. The expression of MDR-1, MRP, cyclin D1 and Bcl-2 increased respectively.

Conclusions: The expression of drug-resistance gene in Tca8113/CDDP was significantly higher than that in Tca8113.